6NK4

KVQIINKKL, crystal structure of a tau protein fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ELECTRON MICROSCOPE
Source details	TECNAI F20 TEM
Temperature [K]	88
Detector technology	CMOS
Collection date	2017-01-19
Detector	TVIPS F416 CMOS CAMERA
Wavelength(s)	0.0251
Spacegroup name	P 61
Unit cell lengths	62.906, 62.906, 4.832
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.240 - 1.994
R-factor	0.2638
Rwork	0.260
R-free	0.29910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5v5b
RMSD bond length	0.010
RMSD bond angle	1.399
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	90.000	27.240	2.150
High resolution limit [Å]	1.994	3.420	2.000
Rmerge	0.244	0.144	0.681
Number of reflections	943	222	174
<I/σ(I)>	2.7
Completeness [%]	97.1	95.3	96.7
Redundancy	6	5.6	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M lithium citrate, 20% PEG3350