6NJW
C-terminal region of the Xanthomonas campestris pv. campestris OLD protein phased with platinum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0716 |
| Spacegroup name | P 43 |
| Unit cell lengths | 65.426, 65.426, 63.759 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.260 - 1.860 |
| R-factor | 0.2128 |
| Rwork | 0.211 |
| R-free | 0.23800 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.823 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.430 | 1.900 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 44393 | |
| <I/σ(I)> | 19.5 | |
| Completeness [%] | 99.9 | |
| Redundancy | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Bis Tris Propane pH 7.0-8.0, 11-26% PEG 3350, and 0.15-0.2 M sodium iodide |
