6NJV

C-terminal region of the Xanthomonas campestris pv. campestris OLD protein phased with iodine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.6531
Spacegroup name	P 43
Unit cell lengths	64.392, 64.392, 63.388
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.390 - 2.300
R-factor	0.2181
Rwork	0.215
R-free	0.27440
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.044
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.390	2.040
High resolution limit [Å]	1.990	1.990
Number of reflections	29501
<I/σ(I)>	13.9
Completeness [%]	99.4
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Bis Tris Propane pH 7.0-8.0, 11-26% PEG 3350, 0.15-0.2 M sodium iodide, and 0.25 M NDSB-195