6NIW
Crystal structure of P[6] rotavirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-12-06 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.691, 76.000, 73.881 |
| Unit cell angles | 90.00, 91.89, 90.00 |
Refinement procedure
| Resolution | 73.840 - 1.550 |
| R-factor | 0.2004 |
| Rwork | 0.199 |
| R-free | 0.23250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vx8 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.546 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.7.1) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 73.840 | 73.840 | 1.630 |
| High resolution limit [Å] | 1.550 | 4.890 | 1.550 |
| Rmerge | 0.042 | 0.024 | 0.476 |
| Rmeas | 0.050 | 0.028 | 0.565 |
| Rpim | 0.027 | 0.015 | 0.300 |
| Total number of observations | 299725 | ||
| Number of reflections | 87191 | 2842 | 12671 |
| <I/σ(I)> | 13.3 | ||
| Completeness [%] | 95.8 | 95.6 | 95.4 |
| Redundancy | 3.4 | 3.3 | 3.4 |
| CC(1/2) | 0.999 | 0.999 | 0.905 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 M HEPES sodium pH 7.5, 1.5 M Lithium sulfate monohydrate |
