6NIS

Crystal structure of a human anti-ZIKV-DENV neutralizing antibody MZ24 isolated following ZPIV vaccination

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	63.850, 66.667, 134.416
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.984 - 2.113
R-factor	0.1665
Rwork	0.165
R-free	0.20030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mtx
RMSD bond length	0.003
RMSD bond angle	0.767
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.800	2.120
High resolution limit [Å]	2.110	2.110
Number of reflections	35154	5276
<I/σ(I)>	12.8
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	291	0.1 M Citric acid/NaOH (pH 4.0), 1M LiCl and 20% PEG 6,000