6NHG

Rhodobacter sphaeroides Mitochondrial respiratory chain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2001-02-03
Detector	MAR CCD 165 mm
Wavelength(s)	1.739
Spacegroup name	I 41 2 2
Unit cell lengths	154.181, 154.181, 598.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.870 - 2.800
R-factor	0.2518
Rwork	0.251
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sqx
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.850
High resolution limit [Å]	2.800	7.560	2.800
Rmerge	0.104	0.044	1.189
Rmeas	0.107	0.045	1.230
Rpim	0.022	0.009	0.310
Number of reflections	167324	4740	4392
<I/σ(I)>	10
Completeness [%]	99.8	98.1	100
Redundancy	23.2	21.8	15.2
CC(1/2)		0.999	0.889

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	277	50 mM MOPS 7.2 pH, 20 mM Ammonium acetate, 20% glycerol. Incubation with 2-5 mol excess of Azoxystrobin. Precipitant 12% PEG4000, 0.5 M KCl, 0.1% DHPC; Protein:PPT ratio 1:0.57