6NG9
Crystal structure of human CD160
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-31 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.572, 51.436, 95.426 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 1.954 |
R-factor | 0.20465 |
Rwork | 0.202 |
R-free | 0.25330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ngg |
RMSD bond length | 0.018 |
RMSD bond angle | 1.950 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.990 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.108 | 0.935 |
Number of reflections | 16516 | 1546 |
<I/σ(I)> | 26 | |
Completeness [%] | 99.8 | |
Redundancy | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M Sodium Formate, 0.1 M Tris, pH8.5 and 30% (W/V) PEG4000 |