6NG3

Crystal structure of human CD160 and HVEM complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	I 41 2 2
Unit cell lengths	87.930, 87.930, 157.698
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.880
R-factor	0.23614
Rwork	0.234
R-free	0.27363
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fhq
RMSD bond length	0.013
RMSD bond angle	1.913
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.930
High resolution limit [Å]	2.880	2.880
Rmerge	0.178	1.552
Number of reflections	7332	364
<I/σ(I)>	14.5
Completeness [%]	99.9
Redundancy	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M HEPES pH7.0, 30% (V/V) Jeffamine ED-2001 pH7.0, and 0.1 M sodium citrate tribasic dihydrate