6NFV

Structure of the KcsA-G77C mutant or the 2,4-ion bound configuration of a K+ channel selectivity filter.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-25
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.987
Spacegroup name	I 4
Unit cell lengths	156.158, 156.158, 76.116
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.918 - 2.130
R-factor	0.2071
Rwork	0.206
R-free	0.24280
Data reduction software	HKL-2000 (v1.0)
Data scaling software	HKL-2000 (v1.0)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.918	2.200
High resolution limit [Å]	2.130	2.130
Number of reflections	51020
<I/σ(I)>	33.74
Completeness [%]	99.3
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG400 , magnesium acetate, sodium acetate