6NFQ
CopC from Pseudomonas fluorescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 77.730, 111.319, 82.222 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.000 - 2.000 |
| R-factor | 0.2296 |
| Rwork | 0.228 |
| R-free | 0.25988 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c9q |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.367 |
| Data reduction software | HKL-2000 (5.8.0230) |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 23030 | |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 2.5 M ammonium sulfate, 0.1 M Bis-Tris propane, pH 6.7, 2 mM yttrium chloride hexahydrate |
