Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 3 1 2 |
| Unit cell lengths | 105.501, 105.501, 35.132 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.132 - 2.151 |
| R-factor | 0.1768 |
| Rwork | 0.175 |
| R-free | 0.20770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gxz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.815 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.29) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.680 | 45.680 | 2.220 |
| High resolution limit [Å] | 2.150 | 8.860 | 2.150 |
| Rmerge | 0.132 | 0.037 | 0.877 |
| Rmeas | 0.135 | 0.038 | 0.899 |
| Rpim | 0.029 | 0.009 | 0.194 |
| Number of reflections | 12203 | 183 | 1027 |
| <I/σ(I)> | 21.6 | ||
| Completeness [%] | 99.7 | 97.7 | 97 |
| Redundancy | 21.8 | 19 | 20.5 |
| CC(1/2) | 0.999 | 0.999 | 0.919 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1M Sodium acetate trihydrate, 30% w/v PEG-mono methylether 5000 |
