6NE9
Bacteroides intestinalis acetyl xylan esterase (BACINT_01039)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-28 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 116.703, 126.553, 110.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.598 - 1.738 |
| R-factor | 0.1846 |
| Rwork | 0.183 |
| R-free | 0.21690 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.927 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.600 | 1.800 |
| High resolution limit [Å] | 1.738 | 1.738 |
| Rmerge | 0.061 | 0.229 |
| Number of reflections | 145030 | 2851 |
| <I/σ(I)> | 15.3 | |
| Completeness [%] | 91.7 | 28.61 |
| Redundancy | 1.9 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 10 mM Ammonium sulfate, 80 mM Sodium Cacodylate pH 6.5, and 21% PEG4000 |
