6NDX
Lysinoalanine cross-linked FlgE dimer from Treponema denticola
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2018-10-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.565, 136.352, 112.184 |
Unit cell angles | 90.00, 96.53, 90.00 |
Refinement procedure
Resolution | 43.100 - 3.040 |
R-factor | 0.2525 |
Rwork | 0.248 |
R-free | 0.31570 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.567 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.100 | 3.130 |
High resolution limit [Å] | 3.040 | 3.040 |
Number of reflections | 28592 | |
<I/σ(I)> | 7.7 | |
Completeness [%] | 96.7 | |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | crystallized in two conditions: (1) 100 mM Tris pH 8.5, 20% (w/v) PEG8000, 200 mM magnesium chloride and (2) 200 mM magnesium acetate, 20% PEG3350 (w/v) |