6NDW
Crystal structure of the wild type D2 domain (A168-T344) of the flagellar hook protein FlgE from Treponema denticola
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-06-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97700 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.104, 61.290, 43.790 |
| Unit cell angles | 90.00, 104.45, 90.00 |
Refinement procedure
| Resolution | 24.838 - 1.725 |
| R-factor | 0.1617 |
| Rwork | 0.159 |
| R-free | 0.18680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wlg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.775 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.725 | 1.730 |
| Number of reflections | 17865 | |
| <I/σ(I)> | 24.5 | |
| Completeness [%] | 99.6 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100 mM HEPES, 2.0M Ammonium sulfate |
