6NDQ
Crystal structure of a LPMO from Kitasatospora papulosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-06 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.453 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.207, 109.574, 42.775 |
| Unit cell angles | 90.00, 113.46, 90.00 |
Refinement procedure
| Resolution | 33.088 - 1.601 |
| R-factor | 0.1982 |
| Rwork | 0.196 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4oy6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.837 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.240 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 39856 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 96.3 | |
| Redundancy | 3.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M MMT buffer pH 4.0 25% PEG1500 |
