6NC1
FtsY-NG high-resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-06 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.367, 75.980, 108.265 |
| Unit cell angles | 90.00, 90.30, 90.00 |
Refinement procedure
| Resolution | 37.990 - 1.598 |
| R-factor | 0.1714 |
| Rwork | 0.170 |
| R-free | 0.20080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cqp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.867 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.990 | 1.600 |
| High resolution limit [Å] | 1.598 | 1.598 |
| Number of reflections | 72072 | |
| <I/σ(I)> | 18.33 | |
| Completeness [%] | 97.8 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297.15 | 0.1 M Bis-Tris pH 7, 30% PEG 2000 MME, 150 mM KBr |
