6NBT

CRISPR Complex Subunit Csm3 from Staphylococcus epidermidis RP62a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0781
Spacegroup name	C 1 2 1
Unit cell lengths	104.612, 50.979, 83.018
Unit cell angles	90.00, 97.05, 90.00

Refinement procedure

Resolution	25.000 - 2.400
R-factor	0.23226
Rwork	0.230
R-free	0.26687
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.292
Data reduction software	XDS (20180126)
Data scaling software	Aimless (0.7.3)
Phasing software	CRANK2 (2.0.180)
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.570	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	16891
<I/σ(I)>	10.3
Completeness [%]	98.2
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	287	PEG 8000, calcium acetate, 2-(N-morpholino)ethanesulfonic acid (MES)