6NAW
Crystal structure of Neisseria meningitidis ClpP E58A activated mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-15 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.122, 119.365, 127.639 |
| Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
| Resolution | 42.759 - 2.399 |
| R-factor | 0.194 |
| Rwork | 0.193 |
| R-free | 0.25210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dkp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.161 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.172 | 0.815 |
| Number of reflections | 115034 | 5753 |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 98.7 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.999 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.2 M potassium thiocyanate 40% MPD |
