6N7U
apo-BDBV223 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | .998 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.975, 75.678, 114.411 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.630 - 2.030 |
| R-factor | 0.2354 |
| Rwork | 0.235 |
| R-free | 0.24970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6n7j |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.710 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.630 | 2.100 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Number of reflections | 37109 | |
| <I/σ(I)> | 12.6 | 2.6 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 6.1 | 5.7 |
| CC(1/2) | 0.950 | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 80 mM sodium citrate tribasic/hydrochloric acid, pH 5.6, 20% PEG300, 200 mM ammonium sulfate, 10% glycerol |
