6N7U
apo-BDBV223 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | .998 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.975, 75.678, 114.411 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.630 - 2.030 |
R-factor | 0.2354 |
Rwork | 0.235 |
R-free | 0.24970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6n7j |
RMSD bond length | 0.003 |
RMSD bond angle | 0.710 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.630 | 2.100 |
High resolution limit [Å] | 2.030 | 2.030 |
Number of reflections | 37109 | |
<I/σ(I)> | 12.6 | 2.6 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 6.1 | 5.7 |
CC(1/2) | 0.950 | 0.850 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 80 mM sodium citrate tribasic/hydrochloric acid, pH 5.6, 20% PEG300, 200 mM ammonium sulfate, 10% glycerol |