6N7Q
Plasmodium falciparum FVO apical membrane antigen 1 (AMA1) bound to cyclised RON2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-05 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.565, 37.975, 72.016 |
| Unit cell angles | 90.00, 91.06, 90.00 |
Refinement procedure
| Resolution | 36.273 - 2.100 |
| R-factor | 0.2088 |
| Rwork | 0.207 |
| R-free | 0.24280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r1a |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.623 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.273 | 2.175 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 19742 | 5980 |
| <I/σ(I)> | 5.75 | |
| Completeness [%] | 100.0 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 15-20% PEG400, 0.1 M Tris pH 8.4 20 % isopropanol, 0.1 M sodium acetate |
