6N76

Crystal Structure of ATPase delta1-79 Spa47 E287R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.954
Spacegroup name	P 1 21 1
Unit cell lengths	43.513, 154.748, 54.965
Unit cell angles	90.00, 109.30, 90.00

Refinement procedure

Resolution	34.895 - 2.892
R-factor	0.1908
Rwork	0.188
R-free	0.24370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5swj
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.900	6.240	2.900
Rmerge	0.212	0.081	1.308
Rmeas	0.239	0.090	1.470
Rpim	0.108	0.040	0.658
Total number of observations	69099
Number of reflections	14991	1512	1457
<I/σ(I)>	3.3
Completeness [%]	98.5	97.2	99.7
Redundancy	4.6	4.5	4.8
CC(1/2)		0.995	0.517

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	298	Tris, Ammonium Acetate, Lithium Sulfate, PEG 4000, MPD