6N6R
Crystal structure of ABIN-1 UBAN in complex with two M1-linked di-ubiquitins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-16 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.900, 88.180, 105.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.000 - 1.950 |
| R-factor | 0.24529 |
| Rwork | 0.243 |
| R-free | 0.27786 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.280 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.760 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.094 | 0.812 |
| Number of reflections | 36800 | 2566 |
| <I/σ(I)> | 15.1 | 3.1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.1 | 7.2 |
| CC(1/2) | 0.997 | 0.853 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 30% v/v PEG-MME550, 0.1 M bis-tris pH 6.5, and 0.05 M calcium chloride dihydrate |
