6N6J
Human REXO2 bound to pAA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.863, 87.566, 124.225 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.669 - 1.317 |
| R-factor | 0.1533 |
| Rwork | 0.153 |
| R-free | 0.17010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6n6i |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.863 | 45.863 | 1.390 |
| High resolution limit [Å] | 1.317 | 4.160 | 1.320 |
| Rmerge | 0.034 | 0.634 | |
| Rmeas | 0.068 | 0.037 | 0.718 |
| Rpim | 0.025 | 0.013 | 0.329 |
| Number of reflections | 118674 | 4074 | 16727 |
| <I/σ(I)> | 16.4 | 15.7 | 1.2 |
| Completeness [%] | 99.6 | 99.8 | 97.4 |
| Redundancy | 7.3 | 7.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 0.2 M sodium malonate (pH 5.5) and 15-20% polyethylene glycol 3350, 25% glycerol |
