6N6F
Vibrio cholerae Oligoribonuclease bound to pGC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 88.715, 88.715, 59.780 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.317 - 1.735 |
| R-factor | 0.1555 |
| Rwork | 0.155 |
| R-free | 0.16990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6n6a |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.180 | 47.180 | 1.830 |
| High resolution limit [Å] | 1.735 | 5.490 | 1.740 |
| Rmerge | 0.027 | 1.033 | |
| Rmeas | 0.056 | 0.028 | 1.080 |
| Rpim | 0.016 | 0.008 | 0.312 |
| Total number of observations | 338345 | ||
| Number of reflections | 28405 | 981 | 4050 |
| <I/σ(I)> | 24 | 20 | 0.8 |
| Completeness [%] | 99.8 | 99.9 | 98.9 |
| Redundancy | 11.9 | 11.1 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M BisTris (pH 5.5), 17% polyethylene glycol 3350, and 20% xylitol |
