6N6D
Vibrio cholerae Oligoribonuclease bound to pAG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 89.734, 89.734, 59.485 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.867 - 1.534 |
| R-factor | 0.1459 |
| Rwork | 0.145 |
| R-free | 0.15780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6n6a |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.235 | 47.235 | 1.620 |
| High resolution limit [Å] | 1.534 | 4.850 | 1.534 |
| Rmerge | 0.028 | 0.894 | |
| Rmeas | 0.076 | 0.029 | 0.936 |
| Rpim | 0.022 | 0.008 | 0.275 |
| Total number of observations | 493098 | ||
| Number of reflections | 41621 | 1432 | 5965 |
| <I/σ(I)> | 19.4 | 20.9 | 0.9 |
| Completeness [%] | 99.8 | 99.8 | 98.8 |
| Redundancy | 11.8 | 11.4 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M BisTris (pH 5.5), 17% polyethylene glycol 3350, and 20% xylitol |
