6N6A
Vibrio cholerae Oligoribonuclease bound to pGG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 89.864, 89.864, 59.622 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.586 - 1.500 |
| R-factor | 0.1474 |
| Rwork | 0.147 |
| R-free | 0.16020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2igi |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3211) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.329 | 47.329 | 1.580 |
| High resolution limit [Å] | 1.500 | 4.740 | 1.500 |
| Rmerge | 0.035 | 1.157 | |
| Rmeas | 0.105 | 0.037 | 1.216 |
| Rpim | 0.030 | 0.011 | 0.369 |
| Number of reflections | 44737 | 1525 | 6440 |
| <I/σ(I)> | 16.3 | 16.1 | 0.7 |
| Completeness [%] | 100.0 | 99.8 | 99.7 |
| Redundancy | 11.8 | 11.4 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M BisTris (pH 5.5), 17% polyethylene glycol 3350, and 20% xylitol |
