6N5U
Crystal structure of Arabidopsis thaliana ScoI with copper bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-10 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.967700 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.650, 80.440, 72.310 |
| Unit cell angles | 90.00, 94.75, 90.00 |
Refinement procedure
| Resolution | 44.750 - 2.660 |
| R-factor | 0.19 |
| Rwork | 0.187 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ggt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.750 | 2.790 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Rmerge | 0.068 | 0.327 |
| Rmeas | 0.085 | 0.408 |
| Rpim | 0.050 | 0.240 |
| Number of reflections | 17555 | 2355 |
| <I/σ(I)> | 5.7 | 1.5 |
| Completeness [%] | 99.0 | 99.6 |
| Redundancy | 2.9 | 2.9 |
| CC(1/2) | 0.996 | 0.932 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 100 mM Hepes pH 7.5, 23 % w/v PEG 3350, 10 % v/v Tacsimate |
