6N5T

Structure of Human pir-miRNA-378a Apical Loop Fused to the YdaO Riboswitch Scaffold

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.116
Spacegroup name	P 31 2 1
Unit cell lengths	113.810, 113.810, 114.990
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	74.834 - 2.787
R-factor	0.1818
Rwork	0.180
R-free	0.19630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qk8
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.834	2.890
High resolution limit [Å]	2.787	2.790
Rmeas	0.059	1.400
Rpim	0.014	0.327
Number of reflections	21687
<I/σ(I)>	27.7	2.1
Completeness [%]	99.1	88.5
Redundancy	20	17
CC(1/2)	1.000	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	297	1.7 M (NH4)2SO4, 0.2 M Li2SO4, and 0.1 M HEPES pH 7.4