6N5Q
Structure of Human pir-miRNA-378a Apical Loop and One-base-pair Fused to the YdaO Riboswitch Scaffold
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-20 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.116 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 114.850, 114.850, 114.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 75.131 - 2.946 |
| R-factor | 0.1674 |
| Rwork | 0.165 |
| R-free | 0.18790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qk8 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.131 | 3.050 |
| High resolution limit [Å] | 2.946 | 2.950 |
| Rmeas | 0.067 | 1.540 |
| Rpim | 0.015 | 0.346 |
| Number of reflections | 18836 | 1884 |
| <I/σ(I)> | 25.5 | 1.9 |
| Completeness [%] | 99.8 | 97.5 |
| Redundancy | 19.4 | 18.9 |
| CC(1/2) | 1.000 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 297 | 1.63 M (NH4)2SO4, 0.272 M Li2SO4, and 0.1 M HEPES pH 7.4 |
