6N5P

Structure of Human pir-miRNA-340 Apical Loop and One-base-pair Fused to the YdaO Riboswitch Scaffold

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.116
Spacegroup name	P 31 2 1
Unit cell lengths	114.800, 114.800, 115.570
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	75.369 - 2.991
R-factor	0.1543
Rwork	0.151
R-free	0.18060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qk8
RMSD bond length	0.005
RMSD bond angle	1.059
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.369	3.100
High resolution limit [Å]	2.991	2.991
Rmeas	0.101	2.020
Rpim	0.016	0.324
Number of reflections	18192	1819
<I/σ(I)>	27.1	2.4
Completeness [%]	99.9	100
Redundancy	38.2	35.7
CC(1/2)	1.000	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	297	1.89 M (NH4)2SO4, 0.214 M Li2SO4, and 0.1 M HEPES pH 7.4