6N5D

Broadly protective antibodies directed to a subdominant influenza hemagglutinin epitope

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9787
Spacegroup name	P 31
Unit cell lengths	153.190, 153.190, 94.160
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.369 - 3.000
R-factor	0.2279
Rwork	0.217
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 1HA0 6n5b & 4HKX
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.369	50.000	3.300
High resolution limit [Å]	3.000	44.369	3.000
Rmerge	0.255		1.199
Rmeas	0.277		1.304
Total number of observations	318259
Number of reflections	49440	0	12268
<I/σ(I)>	9.22		1.84
Completeness [%]	100.0		100
Redundancy	6.437		6.45
CC(1/2)	0.985		0.455

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.2 M sodium bromide, 0.1 M Bis-Tris propane, pH 6.5, 20% w/v PEG3350