6N4P

RQEFEV, crystal structure of the N-terminal segment RQEFEV from protein tau

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	16.590, 11.450, 25.420
Unit cell angles	90.00, 104.24, 90.00

Refinement procedure

Resolution	24.639 - 1.851
R-factor	0.2012
Rwork	0.193
R-free	0.26870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6-residue idealized beta sheet
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.639	24.639	1.980
High resolution limit [Å]	1.850	5.240	1.850
Rmerge	0.160	0.106	0.356
Rmeas	0.196	0.140	0.430
Number of reflections	843	42	147
<I/σ(I)>	4.16	6.05	1.94
Completeness [%]	97.8	93.3	99.3
Redundancy	2.868	2.762	2.932
CC(1/2)	0.971	0.983	0.948

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M ammonium citrate dibasic, 30% PEG3350