6N41

Crystal structure of InvbM.18715.a.KN11: Influenza hemagglutinin from strain A/Netherlands/002P1/1951

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-25
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	70.280, 123.410, 119.970
Unit cell angles	90.00, 95.54, 90.00

Refinement procedure

Resolution	46.274 - 2.500
R-factor	0.1884
Rwork	0.187
R-free	0.23550
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3297)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.274	46.274	2.560
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.092	0.027	0.791
Rmeas	0.106	0.031	0.904
Total number of observations	300573
Number of reflections	70379	806	5165
<I/σ(I)>	12.09	37.63	2.11
Completeness [%]	99.9	97.2	100
Redundancy	4.271	3.891	4.294
CC(1/2)	0.997	0.999	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	289	SEC purified InvbM.18715.a.KN11.PD38309 at 10.4mg/ml (in 25mM TRIS pH 8, 150mM NaCl) was crystallized by sitting drop vapor diffusion at 14C with an equal volume (0.1:0.1uL) of protein:mother liquor (0.1M MMT buffer pH9, 27.5% PEG1500), then cyoprotected in mother liquor+15% ethylene glycol, before being flash-frozen for data collection. Crystal ID 304301a12 data set xee7-4