6N3R

Sheep Galectin-11 (LGALS11) complex with galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-06
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	96.110, 127.430, 140.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.169 - 2.397
R-factor	0.1629
Rwork	0.160
R-free	0.20910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.899
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.10_2148)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.169	2.810
High resolution limit [Å]	2.397	2.710
Number of reflections	68286
<I/σ(I)>	7.3
Completeness [%]	100.0
Redundancy	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	2% v/v Tacsimate, pH 7.0, 0.1 M HEPES, pH 7.5, 20% w/v PEG3350