6N3F

Structure of HIV Tat-specific factor 1 U2AF Homology Motif bound to SF3b1 ULM5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	46.319, 58.710, 125.957
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.314 - 2.099
R-factor	0.1794
Rwork	0.175
R-free	0.21890
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (dev_3051)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.314	37.314	2.160
High resolution limit [Å]	2.099	8.910	2.100
Rmerge	0.087	0.034	0.650
Rmeas	0.098	0.038	0.753
Rpim	0.043	0.017	0.361
Number of reflections	37604	268	1591
<I/σ(I)>	10.1
Completeness [%]	98.4	88.2	97
Redundancy	5	4.9	5
CC(1/2)	0.980	1.000	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277.15	15% PEG 8000, 0.1 M Tris