6N3E

Structure of HIV Tat-specific factor 1 U2AF Homology Motif bound to U2AF ligand motif 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.127
Spacegroup name	P 32 2 1
Unit cell lengths	58.370, 58.370, 97.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.055 - 1.893
R-factor	0.1739
Rwork	0.171
R-free	0.19830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.971
Data reduction software	XDS
Data scaling software	Aimless (0.1.26)
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.055	35.055	1.940
High resolution limit [Å]	1.890	9.080	1.890
Rmerge	0.044	0.025	0.452
Rmeas	0.049	0.028	0.518
Rpim	0.020	0.013	0.243
Number of reflections	15748	182	920
<I/σ(I)>	23.2
Completeness [%]	99.4	98.3	92.1
Redundancy	5.7	4.7	3.9
CC(1/2)	0.999	0.998	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.2 M Magnesium formate dihydrate, 20% PEG 3350