Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9759 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.821, 94.182, 171.112 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.739 - 2.200 |
| R-factor | 0.199 |
| Rwork | 0.198 |
| R-free | 0.22740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fvd |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.704 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.739 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.061 | 0.270 |
| Number of reflections | 59743 | 2672 |
| <I/σ(I)> | 32.9 | 4.2 |
| Completeness [%] | 93.8 | 85.6 |
| Redundancy | 3.1 | 2.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 1.05M ammonium sulfate, 18% Peg 6000, 0.1M imidazole malate |
