6N31
WD repeats of human WDR12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.740, 87.050, 155.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.000 - 2.600 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ow8 side chains modeled with FFAS03/SCWRL |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 76.000 | 76.000 | 2.710 |
| High resolution limit [Å] | 2.600 | 9.000 | 2.600 |
| Rmerge | 0.111 | 0.038 | 0.830 |
| Rmeas | 0.134 | 0.046 | 1.012 |
| Rpim | 0.074 | 0.025 | 0.570 |
| Total number of observations | 65007 | 1650 | 7721 |
| Number of reflections | 21949 | 1650 | 7721 |
| <I/σ(I)> | 7.3 | 22.4 | 1.2 |
| Completeness [%] | 96.7 | 90 | 95.7 |
| Redundancy | 3 | 3.1 | 2.9 |
| CC(1/2) | 0.994 | 0.997 | 0.654 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 25% PEG 3350, 0.2M magnesium chloride, 0.1M Hepes |
