6N2G

Crystal structure of Caenorhabditis elegans NAP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-10
Detector	NOIR-1
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	125.597, 154.868, 92.551
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.796 - 3.003
R-factor	0.2416
Rwork	0.241
R-free	0.25530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.721
Data reduction software	XDS
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	154.868	19.796	3.170
High resolution limit [Å]	3.003	9.500	3.003
Rmerge		0.028	0.666
Rmeas	0.125	0.030	0.720
Rpim	0.047	0.012	0.270
Number of reflections	61500	1136	5141
<I/σ(I)>	13.3	16.3	1.2
Completeness [%]	89.4	88.9	97.4
Redundancy	6.9	6.6	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1 M Sodium HEPES pH 7.5 30% PEG 400