6N22
Crystal structure of mouse Protocadherin-15 EC1-2 BAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-21 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 64 |
| Unit cell lengths | 99.616, 99.616, 58.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.860 - 2.400 |
| R-factor | 0.17326 |
| Rwork | 0.170 |
| R-free | 0.23289 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4apx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.706 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.132 | 0.802 |
| Rpim | 0.047 | 0.311 |
| Number of reflections | 13043 | 646 |
| <I/σ(I)> | 17.833 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.8 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 0.02 M calcium chloride, 0.1 M sodium acetate, 30% (v/v) MPD |
