6N16
Vaccine-elicited NHP FP-targeting neutralizing antibody 0PV-b.01 in complex with HIV fusion peptide (residue 512-519)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.497, 84.204, 84.572 |
| Unit cell angles | 89.88, 111.43, 104.48 |
Refinement procedure
| Resolution | 43.072 - 2.302 |
| R-factor | 0.2243 |
| Rwork | 0.222 |
| R-free | 0.27400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
| Rmerge | 0.080 | 0.029 | 0.330 |
| Rmeas | 0.113 | 0.041 | 0.467 |
| Rpim | 0.080 | 0.029 | 0.330 |
| Total number of observations | 126153 | ||
| Number of reflections | 68478 | 3549 | 3650 |
| <I/σ(I)> | 5.6 | ||
| Completeness [%] | 85.2 | 88.3 | 90.7 |
| Redundancy | 1.8 | 1.9 | 1.9 |
| CC(1/2) | 0.996 | 0.723 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.15 M Lithium sulfate monohydrate, 0.1 M Citric acid pH 3.5, 18% w/v Polyethylene glycol 6,000 |
