6N12

Structure of GTPase Domain of Human Septin 7 at High Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96862
Spacegroup name	P 61
Unit cell lengths	82.815, 82.815, 209.021
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.618 - 2.230
R-factor	0.183
Rwork	0.181
R-free	0.21640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6n06
RMSD bond length	0.008
RMSD bond angle	0.942
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.680	2.290
High resolution limit [Å]	2.230	2.230
Rmerge	0.198	2.260
Rpim	0.045	0.509
Number of reflections	39079	2845
<I/σ(I)>	8.6
Completeness [%]	99.2	98.6
Redundancy	20.4	20.6
CC(1/2)	0.994	0.529

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	10% w/v PEG 8000, 20% v/v ethylene glycol 0.03 M of each CaCl2 and MgCl2, 0.1 M bicine/Trizma base pH 8.5