6N0V
tRNA ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.231, 114.071, 68.200 |
| Unit cell angles | 90.00, 100.36, 90.00 |
Refinement procedure
| Resolution | 46.483 - 2.502 |
| R-factor | 0.2147 |
| Rwork | 0.212 |
| R-free | 0.25610 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.235 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.550 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rpim | 0.052 | 0.436 |
| Number of reflections | 39376 | |
| <I/σ(I)> | 22.2 | 2 |
| Completeness [%] | 97.8 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.996 | 0.827 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M Trimethyl N-oxide, 0.1 M Tris pH 8.5, 20% (w/v) PEG MME 2000 |
