6N0I

2.60 Angstrom Resolution Crystal Structure of Elongation Factor G 2 from Pseudomonas putida.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-20
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	49.434, 79.968, 183.516
Unit cell angles	90.00, 94.21, 90.00

Refinement procedure

Resolution	29.830 - 2.600
R-factor	0.22517
Rwork	0.223
R-free	0.26393
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fn5
RMSD bond length	0.003
RMSD bond angle	1.168
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.078	0.804
Rmeas		0.912
Rpim	0.041	0.427
Number of reflections	44241	2141
<I/σ(I)>	17.3	1.9
Completeness [%]	100.0	100
Redundancy	4.4	4.5
CC(1/2)		0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	Protein: 9.5 mg/ml, 0.01M Tris HCl (pH 8.3); Screen: PACT (C10), 0.2M Magnesium chloride, 0.1M HEPES (pH 7.0), 20% (w/v) PEG 6000.