6N0B

Structure of GTPase Domain of Human Septin 7 at High Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96863
Spacegroup name	C 1 2 1
Unit cell lengths	151.513, 68.866, 150.519
Unit cell angles	90.00, 112.76, 90.00

Refinement procedure

Resolution	62.877 - 1.739
R-factor	0.1754
Rwork	0.174
R-free	0.20280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qnr
RMSD bond length	0.008
RMSD bond angle	0.961
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.850	1.790
High resolution limit [Å]	1.739	1.739
Rmerge	0.088	1.063
Rpim	0.025	0.447
Number of reflections	146736	10760
<I/σ(I)>	14
Completeness [%]	100.0	100
Redundancy	12.2	6.5
CC(1/2)	0.100	0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	10% w/v PEG 4000, 20% v/v glycerol, 0.1 M MOPS/HEPES-Na pH 7.5, and 20mM of each sodium formate, ammonium acetate, trisodium citrate, sodium potassium l-tartrate, sodium oxamate