6MZO

X-ray Structure of an Inactive Zymogen C11 Protease from Parabacteroides distasonis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 2 2 21
Unit cell lengths	73.133, 98.948, 93.943
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.849 - 1.653
R-factor	0.1604
Rwork	0.159
R-free	0.19320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uws
RMSD bond length	0.020
RMSD bond angle	1.770
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.890	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.056	0.612
Rmeas	0.064	0.693
Rpim	0.030	0.318
Number of reflections	40606	1990
<I/σ(I)>	39.8	1.9
Completeness [%]	98.9	98.9
Redundancy	4.6	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.16 M ammonium acetate, 25% PEG 4000, 0.085 M sodium citrate, and 15% glycerol