6MZO
X-ray Structure of an Inactive Zymogen C11 Protease from Parabacteroides distasonis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 73.133, 98.948, 93.943 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.849 - 1.653 |
| R-factor | 0.1604 |
| Rwork | 0.159 |
| R-free | 0.19320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uws |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.770 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.890 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.056 | 0.612 |
| Rmeas | 0.064 | 0.693 |
| Rpim | 0.030 | 0.318 |
| Number of reflections | 40606 | 1990 |
| <I/σ(I)> | 39.8 | 1.9 |
| Completeness [%] | 98.9 | 98.9 |
| Redundancy | 4.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.16 M ammonium acetate, 25% PEG 4000, 0.085 M sodium citrate, and 15% glycerol |
