6MZK

Crystal structure of hemagglutinin from influenza virus A/Pennsylvania/14/2010 (H3N2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-05-30
Detector	NOIR-1
Wavelength(s)	1.00003
Spacegroup name	C 2 2 21
Unit cell lengths	170.720, 394.900, 102.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.556 - 2.500
R-factor	0.2083
Rwork	0.207
R-free	0.23060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yp5
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.560	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.436	8.689
Number of reflections	119566	5835
<I/σ(I)>	15.3	0.7
Completeness [%]	100.0	100
Redundancy	43	41.5
CC(1/2)	0.997	0.366

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M Mes pH 6.5, 1.2M sodium citrate