6MYU

Avian mitochondrial complex II crystallized in the presence of HQNO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9743
Spacegroup name	P 21 21 21
Unit cell lengths	70.029, 83.958, 290.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.780 - 1.970
R-factor	0.199
Rwork	0.199
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yq3
RMSD bond length	0.007
RMSD bond angle	0.922
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((DEV_3150: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.780	2.030
High resolution limit [Å]	1.970	1.970
Rmerge	0.104	1.433
Number of reflections	121715
<I/σ(I)>	8.3	1.2
Completeness [%]	99.7	99.4
Redundancy	6.38	5.52

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	48 g/L PEG3350, 15 mL/L PEG400, 24 mL/L isopropanol, 0.05 M HEPES sodium, 0.01 M Tris-HCl, 1.6 mM manganese chloride, 0.64 mM magnesium chloride, 1.5 mM sodium azide, 0.25 mM sodium EDTA, 10 g/L octyl beta-D-glucoside, undecyl-beta-D-maltoside, |HQNO