6MYS

Avian mitochondrial complex II with Atpenin A5 bound, sidechain outside

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	71.680, 84.424, 293.019
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.750 - 2.370
R-factor	0.212
Rwork	0.211
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yq3
RMSD bond length	0.002
RMSD bond angle	0.547
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((DEV_3150: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.830	2.440
High resolution limit [Å]	2.370	2.370
Rmerge	0.156	1.116
Number of reflections	73996
<I/σ(I)>	7.7	1.7
Completeness [%]	99.7	99.4
Redundancy	9.07	7.97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	47 g/L PEG3350, 30 mL/L PEG400, 23 mL/L isopropanol, 0.05 M HEPES sodium, 0.01 M Tris-HCl, 3.1 mM manganese chloride, 0.62 mM magnesium chloride, 1.5 mM sodium azide, 0.25 mM sodium EDTA, 10 g/L octyl beta-D-glucoside, undecyl-beta-D-maltoside, crystal was soaked with Atpenin A5 after crystallization