6MYG

Mouse Gamma S Crystallin L16 Octamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	61.095, 77.591, 154.052
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.001 - 2.919
R-factor	0.209
Rwork	0.205
R-free	0.28360
RMSD bond length	0.012
RMSD bond angle	1.125
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Number of reflections	16343
<I/σ(I)>	25
Completeness [%]	99.3
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	0.15 M Sodium acetate pH 5.0, 22% PEG MME 550, 8% PEG 300